top of page
AFLIBERCEPT ELISA

AFLIBERCEPT ELISA

Enzyme immunoassay for the quantitative determination of free Aflibercept (Eylea®, Zaltrap®) in serum and plasma.
The solid phase (MTP) is coated by the target molecule, in this case VEGF.
 
Required Volume (µL)10
Incubation Time (min)105
SampleSerum or Plasma
Plate Size96 Tests
Standard Range (ng/mL, 10x)0-2000
Detection Limit (ng/mL)5
Spike Recovery (%)>95
Shelf Life (years)2
 
Intended Use: This kit has been developed for the measurement of drug levels in research, diagnostic and biosimilar uses. It is suitable for Therapeutical Drug Monitoring (TDM) purposes.
 
Eylea® is a trademark of Bayer.
Zaltrap® is a trademark of Sanofi Genzyme.

  • SPECIFICITY

    There is no cross reaction with any other proteins present in native human serum. A screening test was performed with 48 different native human sera. All produced OD450/620 nm values less than the mean OD of standard D. No cross reaction was observed with the other therapeutic antibodies tested (Tocilizumab, Cetuximab, Etanercept, Infliximab, Rituximab and Trastuzumab) at concentrations up to 2 mg/mL. However, Bevacizumab resulted in full cross-reaction as expected. Ranibizumab, another anti-VEGF therapeutic antibody consisted of Fab fragment only, produced a mean OD less than the mean OD of standard D.

     

    SENSITIVITY

    The lowest detectable level that can be clearly distinguished from the zero standard is 5 ng/mL (zero standard +2SD read from the curve) under the above-described conditions. Analytical sensitivity is 5 ng/mL, and corresponding to the detection limit (limit of quantification) of 0.5 µg/mL for undiluted clinical samples because the serum or plasma samples are instructed to be diluted at 1:100 before starting the assay

     

    PRECISION

    Intra-assay CV: <10%.

    Inter-assay CV: <10%.

     

    RECOVERY

    Recovery rate was found to be >95% with native serum and plasma samples when spiked with exogenous Aflibercept.

     

    AUTOMATION

    The ImmunoGuide Aflibercept ELISA is suitable also for being used by an automated ELISA processor.

    Contact Us

    bottom of page